E.coli 2.5% Acetic Acid Beef Carcass
doi: 10.1128/AEM.66.4.1493-1498.2000.
Effects of acid adaptation of Escherichia coli O157:H7 on efficacy of acetic acid spray washes to decontaminate beef carcass tissue
Affiliations
- PMID: 10742232
- PMCID: PMC92013
- DOI: 10.1128/AEM.66.4.1493-1498.2000
Free PMC article
Effects of acid adaptation of Escherichia coli O157:H7 on efficacy of acetic acid spray washes to decontaminate beef carcass tissue
Appl Environ Microbiol. 2000 Apr .
Free PMC article
Abstract
Exposure to low pH and organic acids in the bovine gastrointestinal tract may result in the induced acid resistance of Escherichia coli O157:H7 and other pathogens that may subsequently contaminate beef carcasses. The effect of acid adaptation of E. coli O157:H7 on the ability of acetic acid spray washing to reduce populations of this organism on beef carcass tissue was examined. Stationary-phase acid resistance and the ability to induce acid tolerance were determined for a collection of E. coli O157:H7 strains by testing the survival of acid-adapted and unadapted cells in HCl-acidified tryptic soy broth (pH 2.5). Three E. coli O157:H7 strains that were categorized as acid resistant (ATCC 43895) or acid sensitive (ATCC 43890) or that demonstrated inducible acid tolerance (ATCC 43889) were used in spray wash studies. Prerigor beef carcass surface tissue was inoculated with bovine feces containing either acid-adapted or unadapted E. coli O157:H7. The beef tissue was subjected to spray washing treatments with water or 2% acetic acid or left untreated. For strains ATCC 43895 and 43889, larger populations of acid-adapted cells than of unadapted cells remained on beef tissue following 2% acetic acid treatments and these differences remained throughout 14 days of 4 degrees C storage. For both strains, numbers of acid-adapted cells remaining on tissue following 2% acetic acid treatments were similar to numbers of both acid-adapted and unadapted cells remaining on tissue following water treatments. For strain ATCC 43890, there was no difference between populations of acid-adapted and unadapted cells remaining on beef tissue immediately following 2% acetic acid treatments. These data indicate that adaptation to acidic conditions by E. coli O157:H7 can negatively influence the effectiveness of 2% acetic acid spray washing in reducing the numbers of this organism on carcasses.
Figures
Survival of A and NA E. coli O157:H7 cells initially (0 h) and after 6 h of exposure in BHI-2.5, as enumerated on TSA and SMAC plates. E. coli O157:H7 strains ATCC 43895, ATCC 43889, and ATCC 43890 were categorized as acid resistant, acid adaptable, and acid sensitive, respectively. The horizontal dotted line at 1.30 log10 CFU/ml denotes the minimum detection level. Error bars indicate standard deviations.
Initial reductions of cell numbers and growth or survival of A and NA E. coli O157:H7 ATCC 43895 (acid-resistant strain) cells on lean BCT stored at 4°C following spray washing treatment with W or 2% AA or after no treatment (n = 6). The standard error of the least squares means was equal to 0.14.
Initial reductions of cell numbers and growth or survival of A and NA E. coli O157:H7 ATCC 43889 (acid-adaptable strain) cells on lean BCT stored at 4°C following spray washing treatment with W or 2% AA or following no treatment (n = 6). The standard error of the least squares means was equal to 0.14.
Initial reductions of cell numbers and growth or survival of A and NA E. coli O157:H7 ATCC 43890 (acid-sensitive strain) cells on lean BCT stored at 4°C following spray washing treatment with W or 2% AA or following no treatment (n = 6). The standard error of the least squares means was equal to 0.14.
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